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2018 Agenda and Abstracts | < Previous Next >
2018
OMIG Abstract Purpose: Fungal infections in lamellar keratoplasty is a growing concern. Optisol-GS does not contain an antifungal and supplementation with 0.255 µg/ml has been considered. This study tests the ability of 0.255 µg/ml Amphotericin B (AmpB) in Optisol-GS to eliminate yeast contamination of corneal tissue. Methods: Three isolates of Candida albicans, one of C. parapsilosis, and one of C. glabratawere tested in Optisol with and without AmpB. Corneoscleral rims stored at -80OC were thawed and placed in 10 multiwell plates (4 per plate). The rims were inoculated with 4 respective loads of yeast: 0, 101, 102, and 103 colony forming units in two sets of 5 for five yeasts. One set was filled with Optisol plus AmpB and the other with Optisol only. All 10 plates were incubated at 5°C for 48 hours. After 48 hours, all corneal rims were placed into 10 ml of yeast extract peptone dextrose medium (YPD); a swab culture of each well was plated onto Sabouraud plates; and all plates with the remaining Optisol were incubated at 30°C. Yeast growth was monitored for 10 days. Minimum inhibitory (MIC) and bactericidal (MBC) concentrations were determined. Results: All corneoscleral specimens were positive regardless of fungal load or presence of AmpB. All controls remained negative. MICs and MBCs were equivalent and ranged between 0.5 to 2.0 µg/ml. Conclusion: Amphotericin B at a concentration of 0.255 µg/ml in Optisol-GS at 5°C over 48 hours does not sterilize corneal specimens contaminated with yeast. Disclosure: N
2018 Agenda and Abstracts | < Previous Next >
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